Antimicrobial properties of methanol crude extract of kacangma against crop pathogenic fungi and preliminary report of fungal disease for kacangma

Yong, Lee Kien (2015) Antimicrobial properties of methanol crude extract of kacangma against crop pathogenic fungi and preliminary report of fungal disease for kacangma. [Final Year Project Report] (Unpublished)

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Abstract

Kacangma (Leonurus sibiricus and Leonurus japonicus) is a local medicinal plant in Sarawak, Malaysia. Antimicrobial properties of methanol crude extract of kacangma were examined against six crop pathogenic fungi namely, Fusarium oxysporum, Fusarium solani, Glomerella cingulata, Pyricularia oryza, Colletotrichum sp. and Pestalotiopsis sp.. No significant antimicrobial effects were observed among these tested crop pathogenic fungi. Flavonoid biosynthesis in kacangma plants that planted in three different locations (Batu Kawa residential area, Muara Tuang private farm and UNIMAS greenhouse) was analysed by Two-dimensional paper chromatography and compared. The leaf flavonoid profiles showed distinct polymorphism to one another which indicated the flavonoid biosynthesis in kacangma was environmentally dependent. Meanwhile, preliminary report on fungal disease of kacangma was studied. Leaf spot disease was infected in L. japonicus plant and the suspected causal agent was the unknown fungal species (Fungus B) which was positive in the pathogenicity test. In short, significant antimicrobial effects of kacangma against the crop pathogenic fungi were not showed, the flavonoid biosynthesis in kacangma was environment dependent and the suspected causal agent of leaf spot disease in L. japonicus was Fungus B.

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