Isolation, identification and antibiotic resistance of enterobactericeae from shrimp ponds in Kuala Baram, Miri, Sarawak

Arweena, Iskandar (2014) Isolation, identification and antibiotic resistance of enterobactericeae from shrimp ponds in Kuala Baram, Miri, Sarawak. Masters thesis, Universiti Malaysia Sarawak.

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Abstract

Antibiotics have been widely used to control bacterial pathogens of aquaculture products. Aquaculture is becoming more economically important to increase local fish and shrimp production for food security. Thus, antimicrobial drugs are therefore used to tackle the disease problems and in some cases, they are used as growth promoter. However, prolong usage of the drugs may I ad to severe health problems to human as well as the resistance problem among the bacteria present The present study aims to detdermine the resistancy of the Enterobacteriaceae isolated from water and shrimp from the aquaculture environment. A total of 20 water samples and 40 shrimps were collected from 10 aquaculture pond in a farm in Kuala Baram, Miri, Sarawak. Isolates were first screened by using peR, followed by identification with API 20E Test Kit, and finally conducting the antimicrobial drugs resistant test. Four antimicrobial drugs were used in this study: ampicillin (AMP), chloramphenicol (C), ceftriaxone (eRO), and tetracycline (TE). The results reflected that the 31 bacterial isolates were classified into 4 different families: Enterobacteriaceae. Brucellaceae. Pseudomonadaceae; and Xanthomonadaceae. Antimicrobial resistance test rerevealed that 93.5% of the isolates were resistant to AMP, while others were susceptible to e, eRO and TE. This study revealed the presence of multiple antibiotic resistant bacteria from aquaculture environment.

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