Lactic acid production by Enteroccocus faecium in liquefied sago starch

Cirilo, Nolasco-Hipolito and Octavio, Carvajal Zarrabal and Rubena, Malfia Kamaldin and Ling, Teck Yee and Samuel, Lihan and Kopli, Bujang and Youji, Nitta (2012) Lactic acid production by Enteroccocus faecium in liquefied sago starch. AMB EXPRESS, 2 (53). ISSN 2191-0855

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Abstract

Enterococcus faecium No. 78 (PNCM-BIOTECH 10375) isolated from puto, a type of fermented rice in the Philippines was used to produce lactic acid in repeated batch fermentation mode. Enzymatically liquefied sago starch was used as the sole carbon source, since sago (Metroxylon spp) is a sustainable crop for industrial exploitation. Liquefied sago starch was inoculated with E. faecium to perform the saccharification and fermentation processes simultaneously. Results demonstrated that E. faecium was reused for 11 fermentation cycles with an average lactic acid yield of 36.3 ± 4.71 g/l. The lactic acid production was superior to that of simple batch mode and continuous fermentation in terms of lactic acid concentration. An un-dissociated lactic acid concentration of 1.15 mM affected the productivity of the cells. Work is in progress to maintain and increase the usability of the cells over higher fermentation cycles.

Item Type: Article
Uncontrolled Keywords: Enteroccus faecium, Lactic acid, Repeated batch fermentation, Liquefied sago starch, Cell reuse, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak
Subjects: S Agriculture > S Agriculture (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 07 Jan 2016 07:07
Last Modified: 07 Jan 2016 07:07
URI: http://ir.unimas.my/id/eprint/10104

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