Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002

Muhammad Suhaib, M. H. and Mohd Hasnain, Hussain and Awang Ahmad Sallehin, Awang Husaini and Kopli, Bujang and Dayang Salwani, Awang Adeni and Mohd Azib, Salleh (2013) Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002. Borneo Journal of Resource Science and Technology, 3 (2). pp. 36-39. ISSN 229-9769

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Official URL: http://www.researchgate.net/publication/280813911

Abstract

Characterization of α-amylase gene sequence produced by Bacillus amyloliquefaciens UMAS 1002, a cellulolytic and amylolytic bacilli isolated from sago pith waste is described here. The amyE gene encoding the α-amylase was isolated by polymerase chain reaction. The 1,980 bp of amyE gene corresponding to 660 amino acids showed 99% homology to the α-amylase sequence from Bacillus subtilis X-23 (GenBank: BAA31528). The α-amylase sequence of B. amyloliquefaciens UMAS 1002 (GenBank: KC800929) differs from that of B. subtilis X-23 by 5 amino acids. In silico analysis of α-amylase from B. amyloliquefaciens UMAS 1002 showed similar characteristics compared to α-amylase from B. subtilis X-23

Item Type: Article
Uncontrolled Keywords: Bacillus amyliquefaciens, starch degrading, amylase, in silico, sago waste, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 30 Nov 2015 01:16
Last Modified: 20 Apr 2016 00:39
URI: http://ir.unimas.my/id/eprint/9829

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