Optimization of metabolite extraction from sago leaves tissue

Farhana, Binti Aktar (2013) Optimization of metabolite extraction from sago leaves tissue. [E-LPTA] (Unpublished)

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Abstract

Metabolic profilling have been widely studies in plants especially for highly commercial product. Currently, the study of metabolites in Metroxylon sagu becomes significantly interesting as it is one of the starch-rich sources for many potential industries. Extraction is the most critical step in metabolites profiling. This study was conducted to optimize several parameters in the extraction method of metabolites of sago leaves. The parameters were temperature for extraction, extraction period and extraction times which were tested using solvent extraction method. Methanol chloroform co-solvent was used in extraction with ratio 2 : 1.The data was measured in quantity by weighing the metabolites extracted. From factorial analysis, the result showed there was significant correlation between temperature, extraction period and extraction time towards metabolites extracted in leaves tissue of M. sagu. Optimal condition towards metabolites extraction was at temperature 30oC for 2 hours at first batch extraction.

Item Type: E-LPTA
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2013.
Uncontrolled Keywords: metabolic profiling, Metroxylon sagu, factorial analysis, methanol chloroform co-solvent, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate,postgraduate, Universiti Malaysia Sarawak
Subjects: S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 10 Sep 2015 06:51
Last Modified: 15 Sep 2015 02:15
URI: http://ir.unimas.my/id/eprint/8755

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