Isolation of Oleaginous fungi from various sources in Kuching, sarawak

Ngui, Paulinus Kim Thian (2013) Isolation of Oleaginous fungi from various sources in Kuching, sarawak. [Final Year Project Report] (Unpublished)

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Abstract

The purpose of this project was to isolate and identify oleaginous fungi from various sources in Kuching, Sarawak. Samplings of the oleaginous fungi were performed from food stalls in several markets, soil samples, sago effluent. and ftom sugar cane juices. Samplings were carried out using enrichment medium and Rose Bengal Dichloran Otlorampbenicol Agar. The morphologies and sizes of the selected yeaslS were observed under light microscope and Sudan IV was used to determine the lipid accumulation abilities in the fungi cells. In addition, the oleaginous fungi isolated from Rose Bengal Dicbloran Chloramphenicol Agar underwent Polymerase Chain Reaction and the typing of fungi strains were identified USing partial sequencing of D1/D2 region of 265 rDNA to establish their identities. The resultant sequcoces were compared with those available on the NCBI website database through the BlastN algorithm. From the BiastN analysis, two yeast species; Candida tropicalis and Candida krusei were identified from the twelve cultures.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2013.
Uncontrolled Keywords: Oleaginous fungi, Rose Bengal Dichloran Chloramphenicol Agar (RBDC). Polymerase Chain Reaction (PCR). 26S rONA. Sudan IV, Lipid accumulation, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, Universiti Malaysia Sarawak
Subjects: S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 10 Sep 2015 06:42
Last Modified: 18 Sep 2015 01:38
URI: http://ir.unimas.my/id/eprint/8751

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