Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus

Magdline Sia, Henry Sum and Anna, Andrew (2015) Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus. BioTechnology an Indian Journal, 11 (3). ISSN 0974 - 7435

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Abstract

Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, a short 24-kDa N-terminal of E2 glycoprotein of chikungunya virus was cloned and expressed in E.coli expression system. The E2 recombinant proteinwas expressed as a fusion protein to 6-Histidine for ease of purification. The expression of the 24-kDa recombinant protein was detected by SDS-PAGE and the protein reactivity was evaluated by western blot analysis. The immunogenicity of the 24 kDa proteinwas further tested against human positive and negative sera for chikungunya and dengue. The results showed that the recombinant antigen was able to detect CHIKV positive sera and no cross reactivity was observed with dengue virus positive serum.

Item Type: Article
Uncontrolled Keywords: Chikungunya virus, Glycoprotein, Recombinant antigen, Immunoblot assay, E.coli expression system, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak
Subjects: R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Medicine and Health Sciences
Depositing User: Karen Kornalius
Date Deposited: 28 Jul 2015 07:53
Last Modified: 28 Jul 2015 07:53
URI: http://ir.unimas.my/id/eprint/8406

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