Molecular phylogeny of grouper based on cytochrome oxidase i gene analysis

Muhammad Hafizie, bin Abu Bakar (2014) Molecular phylogeny of grouper based on cytochrome oxidase i gene analysis. [E-LPTA] (Unpublished)

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Abstract

Epinephelus sexfasciatlls (six bar grouper) is a reef-associated species that often known as seafood delicacy in Asian countries. This fish is well-distributed across western Pacific region. Limited molecular data is available for this species, but none came from Malaysia.This study is aimed to sequence COl gene from genus Epinephellls ESM03 followed by determination of the genetic divergence values and phylogenetic analysis. This study involved total genomic DNA extraction, optical density reading, agarose gel electrophoresis, polymerase chain reaction and DNA sequencing as well as data analysis. A total of 496 bp of COl gene from grouper ESM03 had been successfully sequenced and it matched 99% with E. sexfasciatus KC959953.1. Results obtained from this gene showed that there are mutations present in certain base. Pairwise genetic divergence analysis revealed that there is 2.6% of difference between grouper ESM03 and E. sexfasciatus KC959953.l. The phylogenetic trees constructed showed that genus Epinephellis is monophyletic.

Item Type: E-LPTA
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2014.
Uncontrolled Keywords: Epinephelus sexfasciatlls, PCR, COl gene, genetic divergence, monophyletic, Cytochrome oxidase, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate,postgraduate, research, Universiti Malaysia Sarawak
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 20 Jul 2015 01:17
Last Modified: 11 Sep 2015 08:43
URI: http://ir.unimas.my/id/eprint/8336

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