Preliminary Genetic Analysis on Sea Cucumber From Sampadi Island, Sarawak

Nur Natasya, binti Ab Halim (2014) Preliminary Genetic Analysis on Sea Cucumber From Sampadi Island, Sarawak. [E-LPTA] (Unpublished)

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Abstract

Sea cucumbers (Echinodermata: Holothuroidea) are also known as Holothuroids or Holothurians which play important role in coral reef ecosystem. They are well known as a medicinal properties and as a food source. A few molecular studies had been carried out in Malaysia mainly using 16S rRNA gene and COI gene to study molecular phylogeny of Holothuroidea. However, there is no study conducted on Holothuria from Sarawak. Therefore, this study is to sequence COI gene from Holothuria of Sampadi Island and to assess genetic variation between individuals and infer molecular phylogeny of Holothuria. A total of 406 bp of COI gene sequences were obtained from H. leucospilota in this study. Genetic divergence of 1.1% to 2.8% was recorded among H. leucospilota samples from this study and other H. leucospilota sequence data from Genbank thus indicating intraspecific variations between H. leucospilota. Phylogenetic trees constructed using Neighbour Joining (NJ) and Maximum Parsimony (MP) showed monophyletic of the H. leucospilota as they grouped together into a single clade. Limited samples were used in this study. Therefore, more samples from different locations should be used for future work.

Item Type: E-LPTA
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2014.
Uncontrolled Keywords: Sea cucumbers, Holothuria leucospilota, genetic, COI gene, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate,postgraduate,research, Universiti Malaysia Sarawak
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 09 Jul 2015 03:13
Last Modified: 14 Sep 2015 01:05
URI: http://ir.unimas.my/id/eprint/8274

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