Genetic relationship of Jatropha curcas L.from Samarahan, Lundu and Kalimantan detected by random amplified polymorphic DNA (RAPD) markers

Syarifah Nur Liyana, binti Syd Amra (2012) Genetic relationship of Jatropha curcas L.from Samarahan, Lundu and Kalimantan detected by random amplified polymorphic DNA (RAPD) markers. [Final Year Project Report] (Unpublished)

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Abstract

Jatropha curcas L. from family Euphorbiaceae are also known as "Jarak" is getting attention nowadays even in Malaysia also this plant are being study from every aspects. A total of five samples were collected from Samarahan, Lundu and Kalimantan and planted in UNIMAS green house. Fresh Jatropha's leave sample was taken for the DNA extraction, DNA quantification (gel electrophoresis), and peR amplification ofDNA samples to gain the genetic relationship. From the result, a total of48 alleles were generated from 9 OPF primers with 54.2% were polymorphic. OPF-08 primer produced highest polymorphic bands while OPF-17 and OPF-I8 shows all monomorphic bands. Two cluster was constructed which unknown UNIMAS samples was in cluster I while the rest samples were in cluster II, the genetic relationship was analyzed through dendogram using NTSYS-pc 2. I software. An UPGMA dendogram based on the polymorphic bands showed highest genetic similarity between Kg. Rembus I and Kg. Rembus 2 while the lowest similarity is between unknown UNIMAS sample with Kg. Rembus 2. In conclusion, Jatropha curcas L. in Samarahan, Lundu and Kalimantan have quiet high genetic variation among accessions tudied. This study may provide genetic information for next study on Jatropha curcas L. in Sarawak.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2012.
Uncontrolled Keywords: Plant biotechnology, Plant biomass, Jatropha curcas L., Euphorbiaceae, RAPD markers, genetic relationship, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 01 Jul 2015 06:26
Last Modified: 11 Aug 2021 13:27
URI: http://ir.unimas.my/id/eprint/8196

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