Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products

Fasihuddin Badruddin, Ahmad and Mohammed, Akkbik and Zaini Bin, Assim (2012) Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products. Eurasian Journal of Analytical Chemistry, 8 (2). pp. 79-89. ISSN 1306-3057

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Official URL: http://eurasianjournals.com/index.php/ejac/article...


A sensitive and selective reversed phase HPLC method with ultraviolet-visible spectrophotometry detection has been optimized and validated for the simultaneous determination of phenolic compounds, such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as antioxidants, and octyl methylcinnamat (OMC) as UVB-filter in several personal care products. The optimum analytical conditions (280 nm as maximum wave length, 0.8 mL/min as flow rate, pH 3.5, mixture of phase A (acetonitrile) with phase B (water:acetic acid; 99:1; v/v) as mobile phase) were obtained by variation of flow rate, the pH value of phase B, different binary mixtures. The dynamic range was between 1 to 250 mg L-1 with relative standard deviation better than 0.25%, (n=4). Limit of detection for BHA, BHT and OMC were 0.196, 0.170 and 0.478 mg L-1, respectively. While limit of quantification for BHA, BHT and OMC were 0.593, 0.515 and 1.448 mg L-1, respectively. Recovery study was performed by spiking standard of phenolic compounds at four different concentration levels using external standard addition method. The recovery for BHA, BHT and OMC were ranged from 92.1-105.9%, 83.2-108.9% and 87.3-103.7%, respectively. The concentration ranges of BHA, BHT and OMC in 12 commercial personal care samples were 0.13-4.85, 0.16-2.30 and 0.12-65.5 mg g-1, respectively. The concentrations of phenolic compounds in these personal care samples were below than maximum allowable concentration in personal care formulation i.e up to 1% (w/w) for BHA, up to 0.5% (w/w) for BHT and up to 10% (w/w) for OMC

Item Type: Article
Uncontrolled Keywords: Phenolic compounds, personal care products, RP-HPLC-UV/Vis, optimization and validation method, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 24 Jun 2015 04:01
Last Modified: 24 Jun 2015 04:01
URI: http://ir.unimas.my/id/eprint/7961

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