Characterization and expression studies of grb10 genes in zebrafish

Tiong, Francis Ing Chung (2010) Characterization and expression studies of grb10 genes in zebrafish. [Final Year Project Report] (Unpublished)

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Abstract

Grb10 is the member of super family of adaptor proteins which is characterized by the presence of proline rich region, PH domain, BPS region and SH2 domain. Several studies reported a role of Grb10 in regulating the metabolic and mitogenic responses to insulin and IGF-I. Two Grb10 genes namely Grb10a and Grb10b were found in the zebrafish due to duplication. Total RNA was extracted from 10 different organs (eyes, brain, gills, heart, liver, stomach, intestine, muscle, skin and bone) of zebrafish. The extracted RNA is then used to synthesize cDNA template to perform RT-PCR. Interestingly, Grb10a is expressed in all the isolated organs of zebrafish and this expression patterns were similar to the mammalian expression based on the expression profiles.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) - Universiti Malaysia Sarawak, 2010.
Uncontrolled Keywords: Zebra danio, Molecular genetics, Grb10, Grb10a, expression, evolutionary conserved,Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 19 Jun 2015 07:40
Last Modified: 19 Jun 2015 07:40
URI: http://ir.unimas.my/id/eprint/7817

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