Sex identification in canarium odontophyllum miq. (Dabai) using random amplified polymorphic DNA (RAPD) marker

Ting, Jen Ching (2008) Sex identification in canarium odontophyllum miq. (Dabai) using random amplified polymorphic DNA (RAPD) marker. [Final Year Project Report] (Unpublished)

[img]
Preview
PDF
Sex Identification in Canarium odontophyllum Miq. (DABAI) Using Random Amplified Polymorphic DNA (RAPD) Marker 24 pgs.pdf

Download (309kB) | Preview
[img] PDF
Sex Identification in Canarium odontophyllum Miq. (DABAI) Using Random Amplified Polymorphic DNA (RAPD) Marker.pdf
Restricted to Registered users only

Download (1MB)

Abstract

Canarium odontophyllum Miq. is a perennial plant native to Malaysia, Indonesia and Philippines. The pistillate plants bear fruits twice a year and are more preferable than staminate plants. Thus, sex determination in Canarium odontophyllum at seedling stage is very important for crop improvement processes because it accelerates the identification of fruitful pistillate plants. In this study, Random Amplified Polymorphic DNA (RAPD) technique was used to amplify DNA segments, with the objective of finding molecular markers associated to sex determination locus in this species. DNA was isolated from one male and three female Dabai trees and subjected to RAPD analysis. Of 52 RAPD primers, one primer (OPL – 03) produced an approximately 1,000 bp amplification band detectable in all pistillate plants, while absent in the staminate plants. The exclusiveness of this female-specific diagnostic band could be used in a breeding program to screen the gender of Dabai trees long before they reach reproductive maturity, resulting in considerable savings of time and economic resources.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) - Universiti Malaysia Sarawak, 2008.
Uncontrolled Keywords: Molecular microbiology , Diagnostic bacteriology, Canarium odontophyllum Miq., sex determination, Random Amplified Polymorphic DNA (RAPD), molecular markers, diagnostic band, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate
Subjects: S Agriculture > S Agriculture (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 19 Jun 2015 01:28
Last Modified: 19 Jun 2015 01:28
URI: http://ir.unimas.my/id/eprint/7677

Actions (For repository members only: login required)

View Item View Item