Identification of Methane-producing Bacteria from Palm Oil Mill Sludge (POMS) with Solid Cud from Ruminant Stomach

Lau, Seng and Chan, CSW and Husaini, A. A. S. A and Zulkharnain, A. and Apun, K. and Bilung, L. M and Micky, Vincent (2014) Identification of Methane-producing Bacteria from Palm Oil Mill Sludge (POMS) with Solid Cud from Ruminant Stomach. Journal Of Biochemistry, Microbiology And Biotechnology, 1 (1). pp. 23-26.

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Abstract

Biological generation in anaerobic environments such as enteric fermentation and anaerobic waste treatment from agriculture sector are the major contributor of methane gas which has the potential as biogas. The aimed of this study was to identify methane-producing bacteria in anaerobic vessel which contained a mixture of Palm Oil Mill Sludge (POMS) and solid cud taken from the first compartment of cow’s stomach (1:2 and 2:1 ratio) as co-mixture. The comixture was incubated at 50 °C in a 2 L vessel with initial starter of 400 ml and sampling was conducted every 4 weeks interval during 12 weeks of incubation. For specific detection of methanogens, 16S rRNA-cloning analysis was carried out. Methanobrevibacter sp. and Methanosaeta sp. were confirmed to be presence within the 2:1 ratio of co-mixture while only Methanobrevibacter sp. was found in 1:2 ratio of co-mixture on both Week 0 and Week 4. No methanogens were detected for both co-mixtures on Week 8 and Week 12.

Item Type: Article
Uncontrolled Keywords: Palm Oil Mill Sludge (POMS) , solid cud , methane producing bacteria , 16S rRNA-cloning , unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, ,research, Universiti Malaysia Sarawak.
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 24 Jun 2015 00:28
Last Modified: 24 Jun 2015 00:28
URI: http://ir.unimas.my/id/eprint/7376

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