Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia)

Son, Radu and Micky, Vincent and Kasing, Apun and Raha, Abdul Rahim and Patrick Guda, Benjamin and Gulam, Rusul (2002) Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia). Acta Tropica, 83 (2). pp. 169-176. ISSN 0001-706X.

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Bacterial resistance to various antimicrobial agents is common in area with high usage of antibiotics. In this study, the data on antimicrobial susceptibility patterns of Vibrio cholerae O1 from patients during an outbreak period was found to be high but variable rates of multidrug resistance. Thirty-two of 33 V. cholerae isolates harboured the tcp, ctx, zot and ace genes, suggesting their possible roles in the outbreak cases. We analyzed the molecular diversity of a total of 33 strains of V. cholerae O1 isolated from 33 patients between November 1997 and April 1998 using random amplified polymorphic DNA (RAPD) analysis. The 30 typable isolates could be separated into four major clusters containing 5, 17, 2 and 6 isolates, respectively. However, no particular RAPD pattern was predictive of a particular pattern of antibiotic susceptibility. The findings of this study showed that multiple clones seemed to be responsible for cases in the outbreaks in the study area. Cited by 29

Item Type: Article
Uncontrolled Keywords: Vibrio cholerae O1; Antibiotic resistance; Toxin genes; RAPD-PCR; Malaysia , unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education , research, Universiti Malaysia Sarawak.
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QR Microbiology
Q Science > QR Microbiology > QR355 Virology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 05 May 2015 08:16
Last Modified: 05 May 2015 08:16

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