Identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (Neolamarckia cadamba (Roxb.) Bosser)

Wei-Seng, Ho and Shek-Ling, Pang and Julaihi, Abdullah (2014) Identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (Neolamarckia cadamba (Roxb.) Bosser). Physiology and Molecular Biology of Plants, 20 (3). pp. 393-397. ISSN 0974-0430

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/25049467

Abstract

The large-scale genomic resource for kelampayan was generated from a developing xylem cDNA library. A total of 6,622 high quality expressed sequence tags (ESTs) were generated through high-throughput 5’ EST sequencing of cDNA clones. The ESTs were analyzed and assembled to generate 4,728 xylogenesis unigenes distributed in 2,100 contigs and 2,628 singletons. About 59.3 % of the ESTs were assigned with putative identifications whereas 40.7 % of the sequences showed no significant similarity to any sequences in GenBank. Interestingly, most genes involved in lignin biosynthesis and several other cell wall biosynthesis genes were identified in the kelampayan EST database. The identified genes in this study will be candidates for functional genomics and association genetic studies in kelampayan aiming at the production of high value forests.

Item Type: Article
Uncontrolled Keywords: Developing xylem , Expressed sequence tags (EST), Lignin biosynthesis , Neolamarkia cadamba , Wood formation , cDNA library , unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education,undergraduate,postgraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > QC Physics
Q Science > QP Physiology
S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 06 May 2015 08:12
Last Modified: 05 Jun 2021 05:19
URI: http://ir.unimas.my/id/eprint/7134

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