Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in South-East Asia.

Hang, Vu Thi Ty and Holmes, Edward C. and Veasna, Duong and Quy, Nguyen Thien and Hien, Tran Tinh and Quail, Michael and Churcher, Carol and Parkhill, Julian and Cardosa, Mary Jane and Farrar, Jeremy and Wills, Bridget and Lennon, Niall J. and Birren, Bruce W. and Buchy, Philippe and Henn, Matthew R. and Simmons, Cameron P. (2010) Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in South-East Asia. Neglected Tropical Diseases, 4 (7). ISSN 1935-2735

Emergence of the Asian 1 Genotype of Dengue Virus Serotype 2 in Viet Nam_ In Vivo Fitness Advantage and Lineage Replacement in South-East Asia.pdf

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/20651932


A better description of the extent and structure of genetic diversity in dengue virus (DENV) in endemic settings is central to its eventual control. To this end we determined the complete coding region sequence of 187 DENV-2 genomes and 68 E genes from viruses sampled from Vietnamese patients between 1995 and 2009. Strikingly, an episode of genotype replacement was observed, with Asian 1 lineage viruses entirely displacing the previously dominant Asian/American lineage viruses. This genotype replacement event also seems to have occurred within DENV-2 in Thailand and Cambodia, suggestive of a major difference in viral fitness. To determine the cause of this major evolutionary event we compared both the infectivity of the Asian 1 and Asian/American genotypes in mosquitoes and their viraemia levels in humans. Although there was little difference in infectivity in mosquitoes, we observed significantly higher plasma viraemia levels in paediatric patients infected with Asian 1 lineage viruses relative to Asian/American viruses, a phenotype that is predicted to result in a higher probability of human-to-mosquito transmission. These results provide a mechanistic basis to a marked change in the genetic structure of DENV-2 and more broadly underscore that an understanding of DENV evolutionary dynamics can inform the development of vaccines and anti-viral drugs.

Item Type: Article
Uncontrolled Keywords: dengue virus serotype, Thailand, South-East Asia, dengue, diseases, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology > QR355 Virology
R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Institute of Health and Community Medicine
Depositing User: Ramji
Date Deposited: 14 Apr 2015 07:56
Last Modified: 14 Apr 2015 07:56
URI: http://ir.unimas.my/id/eprint/7008

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