A generic assay for whole-genome amplification and deep sequencing of enterovirus A71

Le Van, Tan and Nguyen, Thi Kim Tuyen and Tran, Tan Thanh and Tran, Thuy Ngan and Hoang, Minh Tu Van and Saraswathy, Sabanathan and Tran, Thi My Van and Le, Thi My Thanh and Lam, Anh Nguyet and Jemma L., Geoghegan and Ong, Kien Chai and David, Perera and Vu, Thi Ty Hang and Nguyen, Thi Han Ny and Nguyen, To Anh and Do, Quang Ha and Phan, Tu Qui and Do, Chau Viet and Ha, Manh Tuan and Wong, Kum Thong and Holmes, Edward C. and Nguyen, Van Vinh Chau and Thwaites, Guy and H. Rogier, van Doorn (2015) A generic assay for whole-genome amplification and deep sequencing of enterovirus A71. Journal of Virological Methods. pp. 30-36. ISSN 0166-0934

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Abstract

sometimes fatal hand, foot and mouth disease (HFMD) across the Asia-Pacific region. EV-A71 outbreaks have been associated with (sub)genogroup switches, sometimes accompanied by recombination events. Understanding EV-A71 population dynamics is therefore essential for understanding this emerging infection, and may provide pivotal information for vaccine development. Despite the public health burden of EV-A71, relatively few EV-A71 complete-genome sequences are available for analysis and from limited geographical localities. The availability of an efficient procedure for whole-genome sequencing would stimulate effort to generate more viral sequence data. Herein, we report for the first time the development of a next-generation sequencing based protocol for whole-genome sequencing of EV-A71 directly from clinical specimens. We were able to sequence viruses of subgenogroup C4 and B5, while RNA from culture materials of diverse EV-A71 subgenogroups belonging to both genogroup B and C was successfully amplified. The nature of intra-host genetic diversity was explored in 22 clinical samples, revealing 107 positions carrying minor variants (ranging from 0 to 15 variants per sample). Our analysis of EVA71 strains sampled in 2013 showed that they all belonged to subgenogroup B5, representing the first report of this subgenogroup in Vietnam. In conclusion, we have successfully developed a high-throughput next-generation sequencing-based assay for whole-genome sequencing of EV-A71 from clinical samples

Item Type: Article
Uncontrolled Keywords: Enterovirus A71, Picornavirus, Hand foot and mouth disease, Deep sequencing, Phylogenya, 2015, unimas, university, universiti, Borneo, Malaysia, Sarawak, kuching, samarahan, ipta, education, research, Universiti Malaysia Sarawak
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Medicine and Health Sciences
Depositing User: Ramji
Date Deposited: 09 Mar 2015 00:46
Last Modified: 07 Apr 2015 07:09
URI: http://ir.unimas.my/id/eprint/6562

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