Heterotrophic cultivation of microalgae, scenedesmus dimorphus

Siong, Samantha Ling Chee (2011) Heterotrophic cultivation of microalgae, scenedesmus dimorphus. [Final Year Project Report] (Unpublished)

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Heterotrophic growth of microalgae is an alternative method for biodiesel production. Microalga Scenedesmus sp. is one of the selective strains used as biomass feed source after Chlorella sp. due to its high lipid contents. So far, there is no study focus on the heterotrophic growth of Scenedesmus dimorphus. The aim of this study was to develop technique of heterotrophic cultivation of the microalga. Pre-isolated S. dimorphus was used as heterotrophic microalgae and maintained in Modified Bristol’s Medium (MBM) at room temperature (21-24oC) and then grew at different concentrations of organic nutrients such as glucose, fructose, acetic acid, and proteose peptone and pH. The growth of S. dimorphus under such conditions was investigated. Cell counting using Neubauer haemacytometer was used for growth measurement and neutral lipids was detected using Nile Red staining method. Results showed that S. dimorphus grew better in glucose at pH 5 condition with the highest growth rate reached 0.48. The highest growth rate of the microalga achieved in fructose, acetic acid, and proteose peptone, however were 0.35, 0.33, and 0.21, respectively. Glucose under illumination also supported a mixotrophic growth of S. dimorphus. Accumulation of neutral lipids in heterotrophic S. dimorphus cells was higher than that of the mixotrophic cells. Size of the microalgal cells and cell morphology had also been affected.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2011.
Uncontrolled Keywords: Microalgae--Physiology, Microalgae, Scenedesmus dimorphus, Heterotrophic cultivation, Organic sources, Nile Red, Neutral lipids, Universiti Malaysia Sarawak, UNIMAS, university, university, education, research, Sarawak, Malaysia, kuching, samarahan, borneo, undergraduate
Subjects: Q Science > QR Microbiology
S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 05 Feb 2015 01:05
Last Modified: 06 May 2015 07:38
URI: http://ir.unimas.my/id/eprint/6423

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