Screening for antimicrobial activities in soil fungal isolated from Kubah National Park

Yue, Keong Choon (2012) Screening for antimicrobial activities in soil fungal isolated from Kubah National Park. [Final Year Project Report] (Unpublished)

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Abstract

A study was carried out on soil samples collected from Kubah National Park in order to discover novel antibiotics produced by soil microbes. Twenty one samples of soil were analyzed for antimicrobial producing microbes. The test bacteria used in this study were Escherichia coli, Listeria monocytogenes, Salmonella enteritidis and Klebsiella pneumoniae. Six fungal isolates (P550 AI a iv, P550 AI b iv, P550 AI c iii, P550 AI d iv, P550 AII a iv, P550 AII b iv) showed strong antibacterial activity against different strains of test bacteria during antibiotic activity screening using agar overlay techniques in the preliminary screening followed by secondary screening. A total of 3 fungal isolates (P550AIa, P550AIb, P550AIc) were selected to undergo antibiotics susceptibility testing and characterization. These 3 fungal isolates showed MIC value and produced inhibition zone compared to the positive control (5× dilutions of penicillin-streptomycin solution). The 3 fungal isolates were subjected to thin layer chromatography and bioautography to characterize their antimicrobial active compound. Three fungal isolates were subjected to Polymerase Chain Reaction (PCR) and gel electrophoresis, however only P550AIb showed band on gel electrophoresis. Upon identification, P550AIb was identified as Penicillium verruculosum after being sequenced. All the fungal isolates could be used to produce antimicrobial compound.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2012.
Uncontrolled Keywords: Soil microbiology, Soil fungi, Soil microbial ecology, soil, fungal, antibacterial activity, identification, FSTS, 2012, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 21 Jan 2015 06:17
Last Modified: 18 May 2015 02:58
URI: http://ir.unimas.my/id/eprint/6253

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