Molecular characterization of fruit fly, bactrocera dorsalis complex from different fruit host

Nor Mariana, Abdullah (2012) Molecular characterization of fruit fly, bactrocera dorsalis complex from different fruit host. [Final Year Project Report] (Unpublished)

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Abstract

In this study, 27 adult samples of fruit flies were collected from Bau, Serian, Padawan and Kg. Pinang in Samarahan division. The identification method of Bactrocera dorsalis complex species based on the morphological characteristics of adult are sometimes difficult and got misidentified. The identification of B. dorsalis and B. papayae were done by using the morphological characters such as head, thorax, abdomen and wing pattern of species. A total of 656 base pairs within the mtDNA gene cytochrome oxidase I (COI) was analysed for two species of B. dorsalis complex: 27 individuals ingroup and one outgroup. Phylogenetic relationships among B. dorsalis complex were inferred using Neighbor-Joining, Maximum Parsimony, Maximum Likelihood (ML) and Bayesian analysis. The COI gene sequence of Kimura 2-parameter distance within 27 samples is ranging from 0.0% to 2.2% and between the outgroup, Drosophila melanogaster ranged from 0.0% to 18%. The result of COI suggested that B. dorsalis complex is monophyletic, showed that B. dorsalis and B. papayae are clustered together.

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