Detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture enviroment

Spencer Carlos, anak Bennet (2012) Detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture enviroment. [Final Year Project Report] (Unpublished)

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Abstract

Pseudomonas aeruginosa is a gram-negative rod shape bacterium belonging to family Pseudomonadaceae. The species is a highly adaptable opportunistic pathogen capable of surviving in a variety of environment, including aquaculture environment. Antibiotics are used in the aquaculture environment, and their usage poses a risk of potential transfer of resistance to human and animal pathogens. This study was conducted to isolate P. aeruginosa and identify them based on the PCR detection of oprL gene locus. These species were isolated from fish, prawn and water. Antibiotic resistance pattern was determined by conducting antibiotic susceptibility tests on the isolates. Thirteen isolates of P. aeruginosa were isolated and all of the isolates show resistance to at least one antibiotic. Highest level of resistance was observed against ampicillin and erythromycin and the lowest group was against gentamicin, norflaxin and nalidixic acid.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2012.
Uncontrolled Keywords: Pseudomonas aeruginosa, oprL, aquaculture environment, antibiotic susceptibility test, FSTS, 2012, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 13 Jan 2015 03:19
Last Modified: 25 Jan 2018 03:33
URI: http://ir.unimas.my/id/eprint/6168

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