Prevalence and determination of chloramphenicol-resistant gene in Escerichia coli isolated from aquacultural environment

Mohamad Al Aamin, bin Razali (2012) Prevalence and determination of chloramphenicol-resistant gene in Escerichia coli isolated from aquacultural environment. [Final Year Project Report] (Unpublished)

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Abstract

Aquaculture nowadays seems as a promising sector, both in food production and economic intention. However, recently the public health hazards related to antimicrobial use in aquaculture were become a major concern regarding this sector; including the development and spread of antimicrobial resistant bacteria and resistance genes, and the occurrence of antimicrobial residues in products of aquaculture. Driven by above factor, the main intention ofthis study is to determine the prevalence of antiobiotic-resistance gene selected bacterial strains from aquaculture environment. Escherichia coli isolates from aquaculture origin were grown on selective media, EMB agar. These isolatess were screened for antibiotic resistance by antibiotic susceptibility test using chloramphenicol (30 Jlg) disk and the respective chloramphenicol-resistance gene in the isolates then were further detected by multiplex PCR, using primers C-l, C-2, C-3, C-4 and C-R for cat I, cat II, cat III, and cat IV genes respectively in a single reaction mixture. From all 19 isolates, 7 were resistance to chloramphenicol, and only 4 isolates indicates the presences of cat gene during agarose gel electrophoreses, which is cat II gene. The presence of cat gene in this study indicates the potential chloramphenicol antimicrobial resistance hazard to consumers and aqualculture workers. Further studied should be carried out to determine the origin and the resistance mechanism ofcat gene in the studied isolates.

Item Type: Final Year Project Report
Uncontrolled Keywords: resistance gene, aquaculture, multiplex PCR, E. coli, chloramphenicol, Chloromycetin, FSTS, 2012, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research.
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 20 Oct 2014 03:33
Last Modified: 25 Mar 2015 02:39
URI: http://ir.unimas.my/id/eprint/5075

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