Wen, He and Yanping, Qu and Shukai, Wu and Soh Fong, Lim and Ling, Mo and Hongyu, Mou and Jibin, Song (2025) Quantitative Analysis of Glutathione Utilizing Highly Luminescent Gold Nanoclusters. Journal of Biomedical Materials Research Part A, 113 (10). pp. 1-13. ISSN 1552-4965
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Abstract
Glutathione (GSH), a pivotal regulator of cellular redox homeostasis, requires precise monitoring for clinical diagnostics. This work develops bovine serum albumin-templated gold nanoclusters (BSA-AuNCs) through in situ chloroauric acid reduction, exhibiting orange fluorescence (λem = 610 nm) with 35.8% quantum yield. Systematic evaluation of pH-dependent and time-resolved quenching behavior revealed optimal GSH detection at physiological pH 7.4 within 3 min. The nanozyme demonstrated linear responsivity from 10 nM to 3 μM (Limit of Detection, LOD = 8.7 nM) and validated applicability in biological matrices (urine/serum) with 98.0%–103.3% recovery rates. This photoluminescent platform enables reliable GSH biomarker quantification, advancing point-of-care testing for oxidative stress disorders.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | bovine serum albumin | fluorescence detection | glutathione | gold nanoclusters | quantum yield. |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry R Medicine > R Medicine (General) |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Engineering Faculties, Institutes, Centres > Faculty of Engineering |
| Depositing User: | Soh Fong |
| Date Deposited: | 29 Sep 2025 23:26 |
| Last Modified: | 29 Sep 2025 23:26 |
| URI: | http://ir.unimas.my/id/eprint/49606 |
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