Optimization of Sterilizing Protocol on in vitro Germination of Solanum melongena L. cv. Mini Cluster

Jocarlson, Johny (2022) Optimization of Sterilizing Protocol on in vitro Germination of Solanum melongena L. cv. Mini Cluster. [Final Year Project Report] (Unpublished)

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Abstract

In plant tissue culture, an optimum sterilizing protocol is important and crucial to obtain the best result without any contamination. Contamination during in vitro germination may affect our results since the contaminants will compete for nutrients which may lead to insufficient nutrients for the seeds to grow. In this study, the seeds of S. melongena L. cv. mini cluster is used for in vitro seed germination. Although many studies have been conducted on finding the optimum sterilizing protocol for eggplant seeds, but only a few research that focuses on this specific cultivar, which is S. melongena L. cv. mini cluster. Hence, this study focuses on sterilizing the seeds of S. melongena L. cv. mini cluster with different concentrations of Clorox at different duration to find the optimum sterilizing protocol. The seeds were treated with 15%, 35% and 50% Clorox for 10 minutes, 15 minutes and 20 minutes respectively. After 6 weeks of germination and observation, it is found that seeds sterilized with 70% ethanol and 50% Clorox for 15 minutes have 0% contamination and 100% germination. Despite this research is only focusing on mini cluster cultivar, however other sterilizing protocol still can be used in sterilizing this cultivar and this research can be used on other cultivars as well.

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