Screening of Enzyme Activity of Bacteria Isolated from Water Samples from Wind Cave, Bau

DANIA HUSNA IZZATI, MOHD ZAPRI (2022) Screening of Enzyme Activity of Bacteria Isolated from Water Samples from Wind Cave, Bau. [Final Year Project Report] (Unpublished)

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Microorganisms present in caves were found to have excellent enzymatic activities and biological molecule degradation properties. In addition, it has recently been demonstrated that extracellular enzymes generated by bacteria play a significant role in industrial processes. For instance, bacterial amylases have the ability to be used in a broad range of commercial processes, including those in the food, fermentation, and pharmaceutical sectors. Meanwhile, bacterial xylanases are frequently employed to enhance grain processing and product performance. Therefore, the aim of the research was to screen xylanase and amylase activity in isolated bacteria from a water sample near Wind Cave, Bau. This research goal was achieved by isolating bacteria from water samples. A total of five isolates were successfully isolated from the sample with one isolate showing both amylase and xylanase activity; one isolate for amylase activity; one for xylanase activity when screened with starch and xylan hydrolysis assay respectively. The three isolates which are WD1A, WD1B, and WD2B proceeded with submerged fermentation for crude enzyme production then followed by amylase and xylanase assay, where both enzymes were analysed by using 3′ 5′dinitrosalicylic acid (DNSA). For WD1A, it showed amylase activity within the range of 0.1986 U/ml, while for xylanase activity 0.0803 U/ml. Hence, WD1A was found to be the most active bacteria-generating enzyme. This study has contributed additional knowledge regarding the extracellular enzymes secreted by cave microorganisms. Further studies are needed to establish more on extracellular enzymes produced by microbes isolated from the cave's water sample.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2022.
Uncontrolled Keywords: bacteria, cave, extracellular enzyme, plate assay, DNSA method
Subjects: T Technology > TP Chemical technology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Dan
Date Deposited: 07 Sep 2022 04:52
Last Modified: 16 Feb 2023 08:14

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