Propagation of Vanilla planifolia sp. via Stem Cutting

Syed Zulhakimi, Syed Zulkipli (2022) Propagation of Vanilla planifolia sp. via Stem Cutting. [Final Year Project Report] (Unpublished)

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Abstract

Vanilla planifolia is an orchid species belonging to the Orchidaceae family, which ranked the second largest family of flowering plants and usually cultivated for their priceless pods or fruits, besides various beneficial compounds in the plant. The main issue for growing this plant species is the very slow rate of multiplication, their extensive time taken to fully mature, which is around two to four years. Thus, this project is focused on applying numerous possible approaches towards the in vivo propagation of Vanilla planifolia via stem cutting. The purpose of this study is to identify the most effective hormone to enhance propagation and maximum growth of the plant. Overall, 13 cuttings were applied with ten different treatments including control, BAP (5,10,15 mg/L), GA3 (5,10,15 mg/L) and NAA+BAP (5,10,15 mg/L) The experiment was conducted using a completely randomized design. At 30th day of planting, the number of roots produced, length of roots produced, colour of root, diameter of cuttings and survival rate were observed and recorded. Mean comparison was done for all treatments of each parameter. Nevertheless, all parameters showed insignificant differences compared to control. Further study should be done by considering other factors that could also influence the growth and development of Vanilla planifolia including sterilization procedures, types and concentrations of hormones, growth condition, time frame for experiment etc.

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