Studies on the Essential Oils and Biological Activity of Citrus Spp

FELICITY VALARIE, JAWAN (2019) Studies on the Essential Oils and Biological Activity of Citrus Spp. [Final Year Project Report] (Unpublished)

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Abstract

Leaves and peels of four different Citrus spp (Cilrus hystrix, Citrus microcarpa, Citrus lemon, Citrus reticulata) were subjected to hydrodistillation using Clevenger-type apparatus. The average yields of essential oils in the leaves and peels ranged from 0.08 % to 0.73 % and 0.30 % to 1.01 % respectively. The oils obtained from the fresh leaves and peels sample were analyzed by Gas-chromatography-mass spectrometry (GC-MS). Total of 23 and 20 chemical constituent were identified respectively. The major compounds found in the leaves oils were d-limonene, citronellal, linalool, caryophyllene and humulene. Major compounds found in the peels oils of Citrus spp were d-limonene, beta-bisabolene, alpha-bisabolol and alpha-cadino!. The cytotoxic effect of Citrus spp with brine shrimp given LC;o more than 100 >Jg/mL show that Cilrus spp oils practically non-toxic. All the Citrus spp oils have good antioxidant activity except leaves of Cilrus microcarpa and. Citrus reticulata which have moderate antioxidant activity.

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