Isolation and Identification of Cellobiose Utilizing Yeasts from Local Fruits Surfaces

Nur'Ainnie, Awaluddin (2019) Isolation and Identification of Cellobiose Utilizing Yeasts from Local Fruits Surfaces. [Final Year Project Report] (Unpublished)

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Abstract

The production of fuel ethanol has become crucial among the industrial field as fossil fuels is decreasing over time. Bioethanol is an alternative for fossil fuels as it is environmentally friendly fuel. The current practice to produce ethanol usually require a fermentative organism such as Saccharomyces cerevisiae. In this study, cellobiose fermenting yeasts from local fruits surfaces were isolated on modified YM broth and YM agar. Molecular works such as DNA extraction, agarose gel electrophoresis and Polymerase Chain Reaction using 26S primer were performed to identify the cellobiose fermenting yeasts. From the DNA sequencing results, there are two cellobiose fermenting yeasts, Meyerozyma guilliermondii and Candida tropicalis were identified. This study has demonstrated that cellobiose fermenting yeasts are present on local fruit surfaces.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2019.
Uncontrolled Keywords: Bioethanol, cellobiose, S. cerevisiae, agarose gel electrophoresis (AGE), DNA extraction, Polymerise Chain Reaction (PCR)
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QD Chemistry
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Unai
Date Deposited: 14 Oct 2021 07:44
Last Modified: 14 Oct 2021 07:44
URI: http://ir.unimas.my/id/eprint/36422

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