Molecular sexing of tigers, Panthera tigris

McEwing, R. and Ouitavon, K. and Rovie-Ryan, J. J. and M., Wulansari and Sitam, F. T. and Ogden, R. (2011) Molecular sexing of tigers, Panthera tigris. Conservation Genetics Resources, 4 (2012). pp. 299-301. ISSN 1877-7252

[img] PDF
tigris1.pdf

Download (1MB)
Official URL: https://link.springer.com/article/10.1007/s12686-0...

Abstract

We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.

Item Type: Article
Uncontrolled Keywords: Molecular sex identification Tiger Zinc finger protein Forensics Felids, UNIMAS, University, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, Universiti Malaysia Sarawak
Subjects: Q Science > QL Zoology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Ryan Japning
Date Deposited: 06 Sep 2021 00:57
Last Modified: 06 Sep 2021 00:57
URI: http://ir.unimas.my/id/eprint/36007

Actions (For repository members only: login required)

View Item View Item