The Potential Use of Cassia alata Linn. Extracts to Inhibit Cellulitis Causeing Agent

Gedlyn Steffi, Gerijeh (2018) The Potential Use of Cassia alata Linn. Extracts to Inhibit Cellulitis Causeing Agent. [Final Year Project Report] (Unpublished)

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Abstract

Cassia ala ta Linn. ( C. ala ta Linn.) is a shrub believed to have antimicrobial activity towards certain bacteria including Staphylococcus aureus (S. aureus). Thus, the flower extracts of C. alata Linn. were investigated for their antimicrobial activity on the strains of S. aureus. The extraction of the flower was done in four solvents which were n-Hexane, ethyl-acetate, sterile distilled water and ethanol. After the extract was diluted to the concentration of IO µg/µI, it was shown to best inhibit the growth of S. aureus for the solvent ethyl-acetate and n-Hexane. That shows a higher concentration is the best condition to exhibit the antimicrobial properties for C. alata Linn. extract. The antimicrobial testing were carried out using well diffusion, minimum inhibitory concentration (MIC) and time kill assay. The extracts used were dissolved in four solvents that were n-Hexane, ethanol, ethyl acetate and sterile distilled water. The extracts were extracted using maceration and Soxhlet techniques. The MIC values obtained for solvents extracted using maceration technique were 1200 µg/µL for nHexane and ethanol while 300 µg/µL for ethyl acetate. There were no inhibitions in sterile distilled water. The MIC value for n-Hexane and ethyl acetate extracted using Soxhlet technique was 600 µg/µL and 2400 µg/µL for ethanol and distilled water, respectively. Therefore, there is the potential use of Cassia alata Linn. extracts to inhibit cellulitis causing agent S. aureus

Item Type: Final Year Project Report
Additional Information: Project report (B.Sc.) -- Universiti Malaysia Sarawak, 2018.
Uncontrolled Keywords: Cassia alata Linn., cellulitis, S. aureus, antimicrobial
Subjects: Q Science > Q Science (General)
S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Patrick
Date Deposited: 30 May 2021 16:10
Last Modified: 30 May 2021 16:10
URI: http://ir.unimas.my/id/eprint/35386

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