Isolation and Enumeration of Vibrio cholerae From Fruit Juice

Nadzirah, Kamsiran (2018) Isolation and Enumeration of Vibrio cholerae From Fruit Juice. [Final Year Project Report] (Unpublished)

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, cholerae is a microorganism that are water-borne and could be found in any water body, including water systems in any buildings. This study was conducted in order to detect the presence of W. cholerae in fruit juice from food premises around Kota Samarahan by isolating and enumerating the V cho!erae from fruit juice that obtained from food premises around Kota Samarahan. The method that was applied in this research are Most Probable Number test and Polymerase Chain Reaction. Serial dilution used in Most Probable Number test are 10,10° and 10°. Most of the isolates have the MPN/g value that in range of <3.0 to 53 MPN/g. Total sample(n=30) show no band on the PCR assay with ctxA gene as positive control. It can be conclude that the fruit juices around Kota Samarahan able to be contaminated by V. cho!erae due to several reason.

Item Type: Final Year Project Report
Additional Information: Project report (B.Sc.) -- Universiti Malaysia Sarawak, 2018.
Uncontrolled Keywords: W. cholerae, fruit juice, most probable number, ctxA gene
Subjects: Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Patrick
Date Deposited: 26 Apr 2021 16:55
Last Modified: 17 Aug 2021 06:24

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