Mutation Analysis of the Human Ribosomal Protein S8 (uS8) Gene in Nasopharyngeal Carcinoma (NPC) Cell

Pithrianey Dyllarra, Fernandez (2018) Mutation Analysis of the Human Ribosomal Protein S8 (uS8) Gene in Nasopharyngeal Carcinoma (NPC) Cell. [Final Year Project Report] (Unpublished)

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Abstract

Nasopharyngeal carcinoma (NPC) is one of the major health problem in Malaysia, reported to be the fourth most common cancer among Malaysians and the third most common cancer among Malaysian men. The mechanisms which cause the disease is not fully understood because the understanding about NPC-associated gene is unclear and how ribosomal protein (RP) genes become part of it. In this study, human ribosomal protein S8 (uS8) gene is studied to find out whether or not the mutation of this gene is the cause of differential expression of the ribosomal protein gene which lead to NPC. The objective of this project is to isolate the sequence of uS8 gene in HK1 cell lines and to analyse the sequence of uS8 gene isolated from the HK1 cell lines. In this study, a set of primer is designed by using NCBI primer blast, Oligo Calc, and OligoAnalyzer 3.1. Then, gradient PCR is used to amplify the DNA of interest. However, both objectives are not achieved at the end of this project. There is no amplification of target gene from the gradient PCR as no band is observed from the gel visualization. To conclude, technical error must be reduced and to study about mutation analysis, biological replicates should be conducted instead of studying on one type of cell line only.

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