Tissue Culture of Plukenetia volubilis L.

Farra Amirah, Rusli (2018) Tissue Culture of Plukenetia volubilis L. [Final Year Project Report] (Unpublished)

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Abstract

Plukenetia volubilis (Linn. ) is a Peruvian plant in the Euphorbiaciae family. The present study investigate on the sterilization of P. volubilis leaves and stems. Various levels of bleach with exposure to ethanol were used to surface sterilized the leaves and stems. Surface sterilization using 20% of chlorox concentration for 1-5 minutes with exposure to ethanol had been found effective in controlling the contamination of stem. Calluses were form from sterilize plant stem that gained from embryo of P. volubilis that had been introduced on different basal media to study the effect of different medium (MS and WPM medium). The callus were induced by NAA and BAP. Greenish with light yellowish granulated calluses were successfully induced by the NAA with concentration of 0.5 and BAP of 0.5. Further research on the above-mentioned factors for P. volubilis should be conducted due to its high potential in medical application and commercialized products.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2018.
Uncontrolled Keywords: Sterilization, Leaf explants, Stem explant, Embryo, Callus induction
Subjects: S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Unai
Date Deposited: 07 Apr 2021 01:14
Last Modified: 07 Apr 2021 01:14
URI: http://ir.unimas.my/id/eprint/35029

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