No Association of Latent Membrane Protein 1 (LMP1) Gene Expression of Epstein-Barr Virus and Oxidative Stress Production in Normal Nasopharyngeal Cell

Mohd Aminudin, Mustapha and Sim, Sai-Peng (2021) No Association of Latent Membrane Protein 1 (LMP1) Gene Expression of Epstein-Barr Virus and Oxidative Stress Production in Normal Nasopharyngeal Cell. International Medical Journal, 28 (2). pp. 138-140. ISSN 1341-2051

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Abstract

Objective: To evaluate the effect of LMP1 gene expression on oxidative stress generation in normal nasopharyngeal cell. Material and Methods: LMP1 gene were established in pTracer and pcDNA form. The cell then transfection into normal nasopharyngeal cell line. Level of reactive oxygen species (ROS) will be assessed by measuring the fluorescence generated by the chemical 2',7'-Dichlorohydrofluorescein diacetate (DCFH-DA) for pcDNA and cell rox for pTracer. Association of LMP1 gene expression and oxidative stress level were calculate by comparing mean of pTracer and pcDNA with LMP1 gene and without LMP1 gene by use independent Sample T test. Results: There were no association between LMP1 gene expression in both pTracer LMP1 and pcDNA LMP1 compare to vector control cells in normal nasopharyngeal cell. Conclusion: Our results show that, there are no association of LMP1 gene expression and oxidative stress production in normal nasopharyngeal cell thus support that LMP1 gene.

Item Type: Article
Uncontrolled Keywords: latent membrane protein 1, Epstein-Barr virus, oxidative stress, nasopharyngeal cell.
Subjects: Q Science > QH Natural history > QH426 Genetics
R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Centre for Pre-University Studies
Depositing User: Mustapha
Date Deposited: 30 Mar 2021 02:16
Last Modified: 30 Mar 2021 02:16
URI: http://ir.unimas.my/id/eprint/34944

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