Genotyping system for assessment of community structure of Alexandrium species

Lee, Pei Chi (2014) Genotyping system for assessment of community structure of Alexandrium species. [Final Year Project Report] (Unpublished)

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A molecular approach of automated ribosomal intergenic spacer analysis (ARISA) was undertaken to characterize the distribution and genetic diversity of the dinoflagellates, Alexandrium species in Malaysian waters which contribute to paralytic shellfish poisoning (PSP). ARISA was used to provide fundamental information on the genetic variability among different populations at different spatial and temporal. This approach was used to identify hot spot of Alexandrium species biodiversity and rapidly detect the species in environmental samples. Samples collection was done on Alexandrium species from Kuching area. The clonal cultures were isolated and established. Scanning electron microscopy (SEM), light microscopy and epifluorescence microscopy were used for the morphological observation of Alexandrium species. Genomic DNA was extracted by using CTAB method. Genomic DNA was used for ribosomal genes amplification and sequencing. The ARISA genus- primers was designed in silico and optimized inculture and environmental samples. Environmental clone libraries were generated from different sampling locations. The ARISA profiles of the environmental samples then be genotyped and analysed by bioinformatic tools.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2014.
Uncontrolled Keywords: Paralytic shellfish poisoning (PSP), Dinoflagellates, Alexandrium, Automate Ribosomal Intergenic Spacer Analysis (ARISA), Genus-specific primer.
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Unai
Date Deposited: 10 Feb 2021 05:02
Last Modified: 01 Mar 2024 02:44

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