Development of Pseudo-nitzschia species-specific oligonucleotide primers for ribosomal intergenic spacer analysis

Teng, Sing-Tung and Lim, Hong-Chang and Lim, Po-Teen and Leaw, Chui-Pin (2013) Development of Pseudo-nitzschia species-specific oligonucleotide primers for ribosomal intergenic spacer analysis. In: 9th Malaysia Genetics Congress (Appreciating the Richness of Nature Through Genetics).

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Official URL: http://www.researchgate.net/publication/230779085_...

Abstract

A study was carried out to determine the community structure of Pseudo-nitzschia species based on ribosomal intergenic spacer analysis (RISA). A molecular approach was carried out by using the secondary structure of the internal transcribed spacers (ITSs) transcripts to characterize the genetic variability of Pseudo-nitzschia species. Clonal cultures used were grown in SWII medium at 26°C under 14: 10 hour light: dark photo cycle. Genomic DNA was extracted and used for ITS region amplification and sequencing. The sequences obtained were used to predict the RNA secondary structure. Sequence-structure alignment was performed with related taxa to identify the sequence signatures. Pseudo-nitzschia species- and genus-specific oligonucleotide primers were designed in silico based on the secondary structure information. The designed specific primers were tested for the development of a genotyping system

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Pseudo-nitzschia,ribosomal intergenic spacer analysis, species-specific oligonucleotide primers, genotyping, research, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Institute of Biodiversity and Environmental Conservation
Depositing User: Karen Kornalius
Date Deposited: 05 Dec 2013 08:48
Last Modified: 10 Aug 2015 06:53
URI: http://ir.unimas.my/id/eprint/337

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