Application of the real-time polymerase chain reaction in the detection and enumeration of vibrio cholera from shrimp ponds

Shafillah, binti Sulaiman (2013) Application of the real-time polymerase chain reaction in the detection and enumeration of vibrio cholera from shrimp ponds. [Final Year Project Report] (Unpublished)

[img] PDF (Please get the password from Digital Collection Development Unit, ext: 3932 / 3914)
Application of the real-time (fulltext).pdf
Restricted to Registered users only

Download (3MB)
[img]
Preview
PDF
Application of the real-time (24pgs).pdf

Download (1MB) | Preview

Abstract

Cholera is an enteric disease which causes severe pandemics and it is caused by a bacterium known as Vibrio cholerae. This project focused on the use of real-time PCR to detect and enumerate the presence of V. cholerae from selected shrimp ponds from Asia Aquaculture Sdn. Bhd in Bako Kuching, Sarawak. In this research, repeat in toxin (rtxA) gene was used as the target gene of V. cholerae and the presence of V. cholerae were detected and enumerated of V. cholerae also been done by Real-time PCR. The dynamics of V. cholerae shows a decreasing population during culturing period. The results showed that the V. cholerae population densities was on a decreasing trend with the R2 value in Pond 1 at 0.85, meanwhile R2 value in Pond 14 at 0.68. Thus, it shows a decreasing trend for both shrimp ponds concentration of V. cholerae throughout culturing period. It is believed that real-time PCR assay allows for more rapid detection and identification of V. cholerae which is faster than current conventional assay.

Item Type: Final Year Project Report
Additional Information: Universiti Malaysia Sarawak, (UNIMAS)
Uncontrolled Keywords: Vibrio cholerae, real-time polymerase chain reaction (RT-PCR), rtxA, shrimp pond, FSTS, 2013, Borneo, Universiti Malaysia Sarawak, UNIMAS, university, university, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research
Subjects: Q Science > QH Natural history > QH426 Genetics
T Technology > T Technology (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 30 Jun 2014 01:32
Last Modified: 05 Dec 2017 07:09
URI: http://ir.unimas.my/id/eprint/3274

Actions (For repository members only: login required)

View Item View Item