Phylogenetics of subfamily Murinae (Rodentia: Muridae) inferred from NADH dehydrogenase subunit-2

Muhammad Jamal Rasyidi, Jakiran (2014) Phylogenetics of subfamily Murinae (Rodentia: Muridae) inferred from NADH dehydrogenase subunit-2. [Final Year Project Report] (Unpublished)

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This study examines the relationship of murids at the genera level. The samples for this study were collected from Universiti Malaysia Sarawak Campus (UNIMAS), Kubah National Park, Matang Wildlife Centre, and Bako National Park. DNA was extracted from tissue samples using cetyltrimethylammonium bromide (CTAB) protocol. By using the mitochondrial DNA (mtDNA) NADH dehydrogenase subunit-2 (ND2) gene, the phylogenetic trees from total amount of 10 species constructed using Neighbour-Joining (NJ), Maximum Parsimony (MP), and Maximun Likelihood (ML) analyses. The trees were analyzed using Molecular Evolutionary Genetic Analysis (MEGA) to produce three different topologies. All three tress produced were resolved and showed that murids are monophyletic group. All of the individuals from representative genus present in the study grouped with the individuals from their respective genus. Future study consisting multiple genera from the family should be important to resolve the evolutionary relationship within the family Muridae.

Item Type: Final Year Project Report
Additional Information: Project report (B.Sc.) -- Universiti Malaysia Sarawak, 2014.
Uncontrolled Keywords: Murids, ND2 gene, nucleotide sequence, phylogenetic tree and relationships, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QL Zoology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Patrick
Date Deposited: 28 Oct 2020 10:01
Last Modified: 14 Aug 2021 09:38

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