Optimization of dna extraction from orang utan using mitochondrial cytochrome b gene

Lim, Kennedy Chong Ming (2011) Optimization of dna extraction from orang utan using mitochondrial cytochrome b gene. [E-LPTA] (Unpublished)

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Abstract

This study examines the optimization of DNA extraction from Orang Utans' hair samples. The DNA sequence analyses were carried out using mitochondrial Cytochrome b gene. A total of 8 samples of Orang Utan were used in this study, which were collected from the Matang Wildlife Centre. The methods used were conventional CTAB method and commercial DNA Extraction Kit (Promega). The results showed that extraction was possible using both methods but the DNAs present were contaminated with organic materials. Due to the contaminants and insufficient DNA concentration, optimization of the extracted DNA was not successfully achieved.

Item Type: E-LPTA
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2011.
Uncontrolled Keywords: Optimization, DNA extraction, Orang Utan, mitochondrial cytochrome b gene, conventional CTAB method, 2011, FSTS, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research.
Subjects: Q Science > Q Science (General)
S Agriculture > S Agriculture (General)
S Agriculture > SF Animal culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 05 Dec 2013 07:46
Last Modified: 05 Dec 2017 06:41
URI: http://ir.unimas.my/id/eprint/316

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