Identification of Vtbrio parahaemolyticus from food samples

Vivil, Bakas (2017) Identification of Vtbrio parahaemolyticus from food samples. [Final Year Project Report] (Unpublished)

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The study was conducted to detect the presence of Vibrio parahaemolyticus from collected food samples obtained from Limbang, Sarawak district. The cultural based methods were employed for the identification and detection of V. parahaemolyticus. The Thiosulphate-Citrate-Bile Salt-sucrose (TCBS) agar was used to grow the V. parahaemolyticus cultures. The samples were revived in Luria Burtani broth with 3% of NaCI and incubate overnight at room temperature. Overnight cultures were streaked on TCBS agar plate to obtain the greenish colonies. The fmdings showed that 62 % or sixteen out of twenty six (16/26) of the samples appeared as green colonies on TCBS agar which is positive to V. parahaemolyticus. 38 % appeared as yellow colonies and some of the examined cultures were not growing. The positive samples were seafood and meat. Gramstaining test were conducted to confirm the presence of V. parahaemolytics in sixteen food samples suspected positive to V.parahaemolyticus. The results showed only three out of sixteen samples (3/16) were positive V. parahaemolyticus and thirteen out of sixteen (13/16) samples were other Vibrio sp. and some samples contaminated with other microorganism. The significant of the study is important for surveillance programme for V. parahaemolyticus to minimize foodbome disease due to V. parahaemolyticus.

Item Type: Final Year Project Report
Additional Information: Project report (B.Sc.) -- Universiti Malaysia Sarawak, 2017.
Uncontrolled Keywords: Thiosulphate-Citrate Bile Salt-sucrose (TCBS) agar, Green colony, Vibrio parahaemolyticus, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > Q Science (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Patrick
Date Deposited: 26 Aug 2020 08:56
Last Modified: 02 Sep 2021 13:22

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