Characterization of Starch Degrading Enzymes from Bacillus amyloliquefaciens UMAS 1002 Isolated From Sago Waste

Mohd Hasnain, Md Hussain and Muhammad Suhaib, Mat Hussin and Awang Ahmad Sallehin, Awang Husain (2009) Characterization of Starch Degrading Enzymes from Bacillus amyloliquefaciens UMAS 1002 Isolated From Sago Waste. In: 1st ASEAN Sago Symposium (ASAS 2009), 29 Oct - 30 Oct 2009, Riverside Majestic Hotel, Kuching, Sarawak.

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Abstract

Bacillus amyloliquefaciens UMAS 1002 isolated from sago pith waste was shown to have amylase and cellulase activity. DNA sequence encoding the cellulase gene has previously been isolated with the size of 1527 bp. Screening for amylase gene by polymerase chain reaction using primers based on conserved regions of amylase from B.amyloliquefaciens wild type (J01542.1) has yielded a 2.2kb PCR product. Sequence analysis of this fragment showed potential 14 Open Reading Frames. The longest ORF was 534 bp and the shortest ORF was 168 bp. Alignment of individual ORF using BLASTX showed that one of the ORF with a length of 249 bp has a highest similarity to alpha-amylase from B. amyloliquefaciens FZB2. This fragment is being cloned and expressed in Escherichia coli to determine whether it produces an active amylase.

Item Type: Proceeding (Paper)
Uncontrolled Keywords: Bacillus amyloliquefaciens UMAS 1002, Sago Waste, DNA, PCR, Escherichia coli, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak.
Subjects: S Agriculture > S Agriculture (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 10 Aug 2020 08:29
Last Modified: 10 Aug 2020 08:29
URI: http://ir.unimas.my/id/eprint/31087

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