Random Amplified Polymorphic Dna-PCR Typing of Vibrio parahaemolyticus Isolated from Local Cockles (Anadara granosa)

Lesley Maurice, Bilung and Son, Radu and Abdul Rani, Bahaman and Raha, Abdul Rahim and Suhaimi, Napis and Cheah, Yoke Kqueen and Chandrika, Murugaiah and Yousr, Abdul Hadi and Tunung, Robin and Nishibuchi, Mitsuaki (2015) Random Amplified Polymorphic Dna-PCR Typing of Vibrio parahaemolyticus Isolated from Local Cockles (Anadara granosa). American Journal of Immunology, 1 (1). pp. 31-36. ISSN 1553-619X

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Official URL: http://psasir.upm.edu.my/id/eprint/57076/

Abstract

Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, this study, demonstrated that the local cockles (Anadara granosa) in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. In addition, RAPD-PCR is simple, robust and sensitive typing methods to differentiate the V. parahaemolyticus strains.

Item Type: Article
Uncontrolled Keywords: RAPD-PCR, cockles, Vibrio parahaemolyticus, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 10 Aug 2020 05:08
Last Modified: 10 Aug 2020 05:08
URI: http://ir.unimas.my/id/eprint/31075

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