PHYLOGENETIC ANALYSIS OF TWO FISH GENUS: OSTEOCHILUS AND CYCLOCHEILICHTHYS

Nadia Syahirah, Radzali (2015) PHYLOGENETIC ANALYSIS OF TWO FISH GENUS: OSTEOCHILUS AND CYCLOCHEILICHTHYS. [Final Year Project Report] (Unpublished)

[img] PDF
PHYLOGENETIC ANALYSIS OF TWO FISH 24pgs.pdf

Download (1MB)
[img] PDF (Please get the password from ACADEMIC REPOSITORY UNIT, ext: 082-583932/ 082-583914)
PHYLOGENETIC ANALYSIS OF TWO FISH ft.pdf
Restricted to Registered users only

Download (1MB)

Abstract

construct the phylogenetic tree of Osteochilus spp and Cyclocheilichthys spp using Cytochrome C Oxidase Subunit I (COI) gene and to study the genetic divergence of the Osteochilus spp and Cyclocheilichthys spp using COI gene. Samples were collected from Batang Kerang, Balai Ringin, Serian using three-layered gill net method. A total of three Cyclocheilichthys spp and one Osteochilus spp tissue samples were successfully processed for DNA analysis. The obtained sequences were aligned and edited using MEGA 6.06 program. construct the phylogenetic tree of Osteochilus spp and Cyclocheilichthys spp using Cytochrome C Oxidase Subunit I (COI) gene and to study the genetic divergence of the Osteochilus spp and Cyclocheilichthys spp using COI gene. Samples were collected from Batang Kerang, Balai Ringin, Serian using three-layered gill net method. A total of three Cyclocheilichthys spp and one Osteochilus spp tissue samples were successfully processed for DNA analysis. The obtained sequences were aligned and edited using MEGA 6.06 program. The genetic divergence was analysed using Kimura 2-parameter distances. Neighbor-Joining, Maximum- Likelihood and Maximum-Parsimony trees were constructed to determine the phylogenetic relationships between species. The genetic divergence between species ranged from 0 % (between C. apogon individuals) to 0.163% (between O. vittatus and C. armatus). The phylogenetic analysis suggested that Cyclocheilichthys spp and Osteochilus spp are of different genus. This study was able to determine the monophyletic of Cyclocheilichthys spp and Osteochilus spp based on the COI gene. The genetic divergence was analysed using Kimura 2-parameter distances. Neighbor-Joining, Maximum- Likelihood and Maximum-Parsimony trees were constructed to determine the phylogenetic relationships between species. The genetic divergence between species ranged from 0 % (between C. apogon individuals) to 0.163% (between O. vittatus and C. armatus). The phylogenetic analysis suggested that Cyclocheilichthys spp and Osteochilus spp are of different genus. This study was able to determine the monophyletic of Cyclocheilichthys spp and Osteochilus spp based on the COI gene.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: Osteochilus spp, Cyclocheilichthys spp, COI, phylogenetic analysis, genetic divergence, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Dan
Date Deposited: 09 Jul 2020 07:32
Last Modified: 09 Jul 2020 07:32
URI: http://ir.unimas.my/id/eprint/30348

Actions (For repository members only: login required)

View Item View Item