Establishment of Callus and Induction of Shoot Proliferation in Jatropha curcas L.

Fazira Atiqah, Bt Arifin (2011) Establishment of Callus and Induction of Shoot Proliferation in Jatropha curcas L. [Final Year Project Report] (Unpublished)

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Jatropha curcas L. is a shrub belong to Euphorbiceae family. This species planted in recent years because of its multiple uses especially for biodiesel production. The present study was carried out to improve the available in vitro propagation protocol of J. curcas. An in vitro propagation of J. curcas was achieved through establishment of callus and multiple shoot proliferation. Callus formation were initiated from leaf and hypocotyl explants isolated from 7 -14 days old seedling of J. curcas on MS basal medium supplemented with different concentration of 2,4-D. Excellent growth of callus was obtained in medium supplemented with 0.5 mglL 2,4-D from hypocotyl explants. The callus grew faster during 10 to 30 days of culture then stabilized at slow growth rate. Explant cultured on this medium possessed high regeneration potential and showed bigger and heavier callus by the end of incubation. Shoot proliferation of J. curcas was initiated from lamina explant on medium composition of MS with various concentrations of BAP alone or in combination with IBA. The best respond was found in medium composition ofMS basal medium + 4.0 BAP + 0.5 IBA. The average number of shoot per explant obtained were 12, which were significantly different from the other treatments.

Item Type: Final Year Project Report
Additional Information: Project report (B.Sc.) -- Universiti Malaysia Sarawak, 2011.
Uncontrolled Keywords: Jatropha curcas L., in vitro, callus induction, shoot proliferation, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Patrick
Date Deposited: 02 Jul 2020 03:33
Last Modified: 30 Aug 2023 02:54

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