Transformation of isochorismate synthase in tomato plants

Sidi, Siti Nurul Ain Binti (2013) Transformation of isochorismate synthase in tomato plants. [Final Year Project Report] (Unpublished)


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Isochorismate synthase (ICS) is an important enzyme during the conversion of chorismate into isochorismate in the shikimate pathway. The cDNA was transformed into tomato plant to observe the functional and expression activity. The objective of this study is to insert the ICS gene isolated from Morinda citrifolia into tomato by seed via infiltration method. Mardi Tomato 1 (MT1) seeds were treated with sonication before immersed in infiltration broth on a shaking incubator and subsequently sown into soils. About 887 bp size of ICS cDNA was not successfully transformed into the MT1 plants confirmed by polymerase chain reaction (PCR).

Item Type: Final Year Project Report
Additional Information: Universiti Malaysia Sarawak, (UNIMAS)
Uncontrolled Keywords: ICS, infiltration, tomato, Agrobacterium tumefaciens, seed, FSTS, 2013, Universiti Malaysia Sarawak, UNIMAS, university, universiti, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research
Subjects: A General Works > AC Collections. Series. Collected works
S Agriculture > SB Plant culture
T Technology > T Technology (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 22 May 2014 04:12
Last Modified: 16 Mar 2015 03:24

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