Isolation and characterization of Pyricularia oryzae isolated from lowland rice in Sarawak, Malaysian Borneo

Nor Ain, Hussin and Yeo, Freddy Kuok San and Nur Karimah, Mohamad and Nur Najwa, Hamsein and Vu, Thanh Tu Anh and Hung, Hui Chung and Lee, San Lai (2020) Isolation and characterization of Pyricularia oryzae isolated from lowland rice in Sarawak, Malaysian Borneo. Malaysian Journal of Microbiology, 16 (1). pp. 58-67. ISSN 1823-8262

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Aims: Rice blast disease caused by Pyricularia oryzae is one of the major biotic diseases of rice in Sarawak, Malaysian Borneo. This study aims to isolate and characterize rice blast fungus obtained from infected leaf collected from four different divisions in Sarawak, viz, Miri, Serian, Sri Aman, and Kuching. Methodology and results: Twelve succeeded isolates were pre-identified as P. oryzae by morphological characteristics of spores, followed by verification through (internal transcribed spacer) ITS sequencing. The isolates were evaluated for morphological characteristics, growth rate and sporulation rate, which were grown on two types of media, (filtered oatmeal agar) FOMA and (potato dextrose agar) PDA. Morphological characterization showed that the colony surface of the different isolates varied from smooth and fluffy to rough and flattened mycelia; some were with the present of concentric rings, and some with aerial mycelia. The growth rate and sporulation rate of each isolate varied based on types of media used. Most of the isolates grew faster on PDA than on FOMA but produced higher number of spores on FOMA as compared to PDA. Conclusion, significance and impact of study: This preliminary study showed that there were variations observed based on morphological and physiological characterization for the different isolates collected in Sarawak, Malaysian Borneo. This study is the first step towards understanding variation in the population of P. oryzae from Sarawak.

Item Type: Article
Uncontrolled Keywords: Pyricularia oryzae, Sarawak rice, morphological characteristics, growth rate, sporulation rate, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, Centre for Academic Information Services,
Subjects: Q Science > Q Science (General)
Q Science > QK Botany
S Agriculture > S Agriculture (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 07 Feb 2020 07:54
Last Modified: 05 Apr 2021 04:48

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