Cytogenetics Analysis of Gills Cell Derived from Rasbora sarawakensis.

Fatin Nadiah, Binti Md Nazri (2017) Cytogenetics Analysis of Gills Cell Derived from Rasbora sarawakensis. [E-LPTA] (Unpublished)

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Abstract

Rasboras are members of a group of small minnow-type of freshwater fish that belongs to Cyprinidae family. Currently, Rasbora establishes the most species-rich genus in the cyprinid subfamily Danioninae with 77 species. The characterization and chromosomal configuration of Rasbora species still remains limited due to cryptic species diversity. Hence, in order to clarify unresolved issues of Rasbora characterization and chromosomal configuration, gills from Rasbora sarawakensis were isolated and trypsinized before tissue were harvested and cultured. Through this, cell suspensions were observed and times of culture were optimized. Cell monolayer were observed in T25 flask and harvested for karyotyping purpose. Although Gbanding was performed, nevertheless due to limited time frame, specific karyotype was not deduced. Although cytogenetics analysis was performed partially, the impact of this study should not be undermined since it may provide critical evidence in the unsettled issue of Rasbora chromosomal configuration.

Item Type: E-LPTA
Additional Information: Project Report (BSc.) - Universiti Malaysia Sarawak , 2017.
Uncontrolled Keywords: Rasbora spp, cytogenetic analysis, epidermal tissue, chromosomal configuration, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, , research, Universiti Malaysia Sarawak.
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 19 Nov 2019 08:16
Last Modified: 19 Nov 2019 08:16
URI: http://ir.unimas.my/id/eprint/27973

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