Assessment of Staphylococcus aureus in Sports Facilities at Selected Gymnasium in Kota Samarahan and Kuching, Sarawak.

Aina Ariffah, Binti Mohd Rozali (2017) Assessment of Staphylococcus aureus in Sports Facilities at Selected Gymnasium in Kota Samarahan and Kuching, Sarawak. [Final Year Project Report] (Unpublished)

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Abstract

Staphylococcus aureus is a type of bacteria that is commonly found in nose, respiratory tract and on the skin. S. aureus can cause serious infection to human by a contact with an object that is well contaminated with S. aureus. Sports equipment at the gym may become the medium of transmission of the infection of Staphylococcus. Therefore, the aim of this study is to assess the presence of S. aureus on the surface of sports facilities in selected gymnasium at Kuching and Kota Samarahan, Sarawak. Forty-two swab samples were collected randomly from sports equipment in the selected gymnasiums. Swab samples were streaked on Baird Parker agar plate to isolate S. aureus. Further identification of the isolates were performed for 31 samples by using Gram staining and catalase test. Genomic DNA extraction was carried out by the boiled cell extraction method. Specific PCR was done to confirm the presence of S. areus by targeting the nuc gene at 270 bp. The findings showed 31 out of 42 samples (73.81 %) were positive S. aureus. This study shows that gymnasium equipment are the reservoir for the survival of S. aureus. In conclusion, the gym equipment are contaminated with S. aureus and this study has provided preliminary research on microbial contamination.

Item Type: Final Year Project Report
Additional Information: Project Report (BSc.) - Universiti Malaysia Sarawak , 2017.
Uncontrolled Keywords: Staphylococcus aureus, gym, Baird-Parker agar, sports equipment, infection, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 19 Nov 2019 07:48
Last Modified: 19 Nov 2019 07:48
URI: http://ir.unimas.my/id/eprint/27962

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