Establishment of Axenic Explants and Callus Culture of Annona muricata Linn.

Maizatul Izzati, Binti Shuib (2017) Establishment of Axenic Explants and Callus Culture of Annona muricata Linn. [Final Year Project Report] (Unpublished)

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Abstract

Soursop (Annnona muricata L.) has been commercially exploited for its medicinal values. Large scale planting of the species requires huge number of planting stock that could not be achieved by using seeds as the seed possess some degree of dormancy. Thus, vegetative propagation by tissue culture holds great potential to produce quality planting stock. This study was designed to determine a working protocol for axenic leaf explants establishment and callus induction of A. muricata. For surface sterilization experiment, the leaves were cut and washed with Tepol detergent, followed by immersion in 200ml of 70% ethanol for 30 seconds, before they were soaked in different concentration of Chlorox® for different exposure times. The second experiment aimed at looking on the best 2,4-D concentration for callus induction. The results showed that 15% Chlorox® with 30 minutes exposure produced the 100% axenic explants. Secondly, leaf explants placed on MS + 4.0mg/ml 2,4-D + 1.0 mg/ml BAP and MS + 6.0 mg/ml 2,4-D + 1.0 mg/ml BAP were the most number of explants producing callus although no significance among the treatment.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2017.
Uncontrolled Keywords: Establishment axenic explants, callus induction, Annona muricata L., micropropagation, 2,4-dichlorophenoxy acetic acid, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 15 Nov 2019 02:40
Last Modified: 15 Nov 2019 02:40
URI: http://ir.unimas.my/id/eprint/27828

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